Email: info@neweastbio.com   |  Call: 6109452007

CD33 (DM77) Rabbit mAb

Anti-CD33 (DM77) Rabbit Monoclonal Antibody
5/5

$445.00

Cat.#:  28395

   Size:   100 μg

In Stock

          Product Description          
Cat.#:   28395
Product Name:   Anti-CD33(DM77) Rabbit Monoclonal Antibody
Synonyms:   CD33;SIGLEC3;gp67
Description:    Anti-CD33 antibody(DM77) Rabbit Monoclonal Antibody
Background:    Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state. Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans. Upon engagement of ligands such as C1q or syalylated glycoproteins; two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK. These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6:SHP-1 and PTPN11:SHP-2. In turn; these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules. One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase:PI3K.
Applications:    ELISA; Flow Cyt
Recommended Dilutions:    ELISA 1:5000-10000; Flow Cyt 1:100
Host Species:    Rabbit
Isotype:    Rabbit IgG
Purification:    Purified from cell culture supernatant by affinity chromatography
Species Reactivity:    Human CD33
Constituents:    Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).

Figure 1. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CD33 protein, hFc-His tagged protein 11166 can bind Rabbit anti-CD33 monoclonal antibody  (clone: DM77) in a linear range of 1-100 ng/ml.

Figure 2. Expi 293 cell line transfected with irrelevant protein  (A) and human CD33  (B) were surface stained with Rabbit anti-CD33 monoclonal antibody 1µg/ml  (clone: DM77) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.

Figure 3. Flow cytometry data of serially titrated Rabbit anti-CD33 monoclonal antibody  (clone: DM77) on Expi 293 cell line transfected with human CD33. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
          Publications