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Human CD47 Full Length Protein

Human CD47 Full Length Protein-Synthetic Nanodisc


Cat.#:  11020

   Size:   50 μg

3-4 weeks

          Product Description          
Cat.#:     11020
Product Name:   Human CD47 Full Length Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   IAP; MER6; OA3
Target:    CD47
UNIPROT ID:    Q08722
Description:    Human CD47 full length protein-synthetic nanodisc
Background:     A membrane protein involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes.
Species/Host:    HEK293
Molecular Weight:    The human full length CD47 protein has a MW of 35.2 kDa
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure1. Elisa plates were pre-coated with Flag Tag CD47-Nanodisc (0.2 µg/per well). Serial diluted anti-CD47 monoclonal antibody (28058) solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-CD47 monoclonal antibody binding with CD47-Nanodisc is 2.959ng/ml.

Figure2. Human CD47-Nanodisc, Flag Tag on SDS-PAGE