| Cat.#: 11107 |
| Product Name: Human CMKLR1 Full Length Protein |
| Size : 10 µg; 50 µg and 100 µg |
| Synonyms: CHEMERINR; ChemR23; DEZ; RVER1 |
| Target: CMKLR1 |
| UNIPROT ID: Q99788 |
| Description: Human CMKLR1 Full Length Protein-Synthetic Nanodisc |
| Background: Receptor for the chemoattractant adipokine chemerin/RARRES2 and for the omega-3 fatty acid derived molecule resolvin E1. Interaction with RARRES2 induces activation of intracellular signaling molecules, such as SKY, MAPK1/3 (ERK1/2), MAPK14/P38MAPK and PI3K leading to multifunctional effects, like, reduction of immune responses, enhancing of adipogenesis and angionesis. Resolvin E1 down-regulates cytokine production in macrophages by reducing the activation of MAPK1/3 (ERK1/2) and NF-kappa-B. Positively regulates adipogenesis and adipocyte metabolism. Acts as a coreceptor for several SIV strains (SIVMAC316, SIVMAC239, SIVMACL7E-FR and SIVSM62A), as well as a primary HIV-1 strain (92UG024-2). |
| Species/Host: HEK293 |
| Molecular Weight: The human full length CMKLR1 protein has a MW of 42.3 kDa |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Protein Families: Druggable Genome, GPCR, Transmembrane |
| Protein Pathways: N/A |
Figure 1. Elisa plates were pre-coated with Flag Tag CMKLR1-Nanodisc (0.2μg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with CMKLR1-Nanodisc is 2.107ng/ml. | 
Figure 2. Human CMKLR1-Nanodisc, Flag Tag on SDS-PAGE |
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