| Cat.#: 28451 |
| Product Name: Anti-CSF1R(DM106) Rabbit Monoclonal Antibody |
| Synonyms: CSF1R;C-FMS;CD115;CSFR;FIM2;FMS;M-CSFR |
| Description: Anti-CSF1R antibody(DM106) Rabbit Monoclonal Antibody |
| Background: The protein encoded by this gene is the receptor for colony stimulating factor 1; a cytokine which controls the production; differentiation; and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a tyrosine kinase transmembrane receptor and member of the CSF1:PDGF receptor family of tyrosine-protein kinases. Mutations in this gene have been associated with a predisposition to myeloid malignancy. The first intron of this gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction. Alternative splicing results in multiple transcript variants. Expression of a splice variant from an LTR promoter has been found in Hodgkin lymphoma (HL); HL cell lines and anaplastic large cell lymphoma. |
| Applications: ELISA; Flow Cyt |
| Recommended Dilutions: ELISA 1:5000-10000; Flow Cyt 1:100 |
| Host Species: Rabbit |
| Isotype: Rabbit IgG |
| Purification: Purified from cell culture supernatant by affinity chromatography |
| Species Reactivity: Human CSF1R |
| Constituents: Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization. |
| Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). |
Figure 1. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CSF1R protein,His tagged protein 11190 can bind Rabbit anti- CSF1R monoclonal antibody (clone: DM106) in a linear range of 0.6-60 ng/ml. | 
Figure 2. Flow cytometry analysis with Anti-CSF1R (DM106) on Expi293 cells transfected with human CSF1R(Blue histogram)or Expi293 transfected with irrelevant protein (Red histogram). |
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