$415.00
Cat. #: | 10465 | ||
Name: | Dio-Ox-LDL | ||
Size: | 0.5 mg | ||
Purity: | 98% (Co-migrates with reference on agarose gel electrophoresis) | ||
Concentration: | Minimum 1.0 mg/ml protein | ||
Description: | Human Dio-Labeled Oxidized Low Density Lipoprotein | ||
Background: | Purified human LDL (d=1.019 – 1.063 g/mL) is oxidized with copper (II) sulfate and labeled with a derivative of DiO (3,3′- dioctadecyloxacarbocyanine). The resultant product is exhaustively dialyzed against phosphate buffered saline, (pH 7.4), sterilized by membrane filtration and then aseptically packaged in a solution containing phosphate-buffered saline at pH 7.4 and 0.2 mM EDTA. Each lot is evaluated on a murine macrophage cell line for fluorescence uptake. | ||
Storage & Stability: | Dio-Ox-LDL is stable for 6 weeks after receipt when handled aseptically and stored at 2-8°C (Don’t Freeze). Note: After prolonged storage, some precipitate may be observed. This is normal for the product. Spin in centrifugation at 5000×g for 10 minutes before using. |