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ERBB2 pAb

Anti-ERBB2 Rabbit Polyclonal Antibody
5/5

$219.00

Cat.#:  21202

   Size:   100 μl

In Stock

          Product Description          

ErbB2 Polyclonal Antibody

Cat. #:  21202
Gene Symbol:  ERBB2/HER2/Neu
Description:   Anti-ErbB2 Rabbit Polyclonal Antibody
Background:   The ErbB family is composed of four plasma membrane-bound receptor tyrosine kinases. Amplification or over-expression of the ERBB2 gene occurs in approximately 30% of breast cancers. HER2 is encoded by ERBB2, a known proto-oncogene located at the long arm of human chromosome 17(17q21-q22). HER2 proteins have been shown to form clusters in cell membranes that may play a role in tumorigenesis.
Immunogen:   A synthetic peptide from the central of ErbB2 protein.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:500-1:5000
WB: 1:200-1:2000
IHC: 1:20-1:200
Concentration:   0.2 mg/ml
Host Species:   Rabbit
Format:   Liquid
Clonality:   Polyclonal
Isotype:   IgG
Purity:   Purified from serum
Preservative: No
Constituents:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes ErbB2 of vertebrates.
Storage Conditions:  Store at -20°C. Avoid repeated freezing and thawing.
Western blot:  

Western blot analysis of ErbB2 protein. Purified full length His-tagged ErbB2 protein (Cat. #10208) was blotted with Anti-ErbB2 rabbit polyclonal antibody (Cat. #21202).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Anti-ErbB2 polyclonal antibody (Cat. #21202). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4 °C. A HRP-conjugated Goat anti-Rabbit IgG (1:50) was used as secondary antibody.
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