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ERBB2(D769H)

Anti-ERBB2(D769H) Mouse Monoclonal Antibody
5/5

$495.00

Cat.#:  26381

   Size:   100 μl

In Stock

          Product Description          
ERBB2(D769H)
Cat. #:  26381
Gene Symbol:  ERBB2/HER2/Neu/c-Neu
Description:   Anti-ERBB2(D769H) Mouse Monoclonal Antibody
Background:   The HER-2/neu oncogene, a member of the epidermal growth factor receptor or erb-B gene-like family, encodes a transmembrane tyrosine kinase receptor that mediates extracellular signals activated by epidermal growth factors. Her2 abnormal has been strongly associated with many malignant tumors, especially with breast cancers. The expression level of Her2 is an important criteria in clinic evaluating of the progression of breast cancer.
Immunogen:   A synthetic peptide from the internal region of ERBB2 which includes the mutation of D769H, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:    ELISA: 1:1000-1:2000 WB: 1:500-1:1000 IF: 1:25-1:50
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Preservative: No
Constituents:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes D769H mutant, but not wild type ERBB2 of vertebrates.
Storage Conditions:   Store at -20°C. Avoid repeated freezing and thawing
Western blot:   Western blot analysis of recombinant ERBB2(D769H) and wild type proteins. Purified His-tagged ERBB2(D769H) protein (lane 1) and corresponding wild type protein (lane 2) were blotted with Anti-ERBB2(D769H) monoclonal antibody (Cat. #26381). Immunofluorescence:   Immunofluorescence of cells expressing ERBB2 proteins with Anti-ERBB2(D769H) antibody. HEK293T cells were transfected with pCDNA3-GFP-ERBB2 (WT) plasmid (left column) or pCDNA3-GFP-ERBB2(D769H) plasmid (right column), then fixed and stained with Anti-ERBB2(D769H) monoclonal antibody (Cat. #26381).
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