NewEast Biosciences pioneered the research and development of the antibodies for GTPases and mutated Oncogene ten years ago. GTPases involve (1) signal transduction in response to activation of cell surface receptors, including transmembrane receptors such as those mediating taste, smell and vision, (2) protein biosynthesis at the ribosome, (3) regulation of cell differentiation, proliferation, division and movement, (4) translocation of proteins through membranes, (5) transport of vesicles within the cell, and vesicle-mediated secretion and uptake, through GTPase control of vesicle coat assembly. An oncogene is a gene that has the potential to cause cancer.
We offer three unique categories of antibodies, which (1) recognize only the active configuration of GTPase (not the inactive one), (2) mutated Oncogene (not mild type) and (3) have super affinity for cAMP and cGMP (no acetylation required). We have over one thousand peer reviewed articles cited our products.
$495.00
Cat. #: 26080 |
Gene Symbol: ERBB2/HER2/Neu |
Description: Anti-ErbB2(V777A) Mouse Monoclonal Antibody |
Background: The ErbB family is composed of four plasma membrane-bound receptor tyrosine kinases. Amplification or over-expression of the ERBB2 gene occurs in approximately 30% of breast cancers. HER2 is encoded by ERBB2, a known proto-oncogene located at the long arm of human chromosome 17(17q21-q22). HER2 proteins have been shown to form clusters in cell membranes that may play a role in tumorigenesis. Mutations of ErbB2 protein, including V777A, have been indicated in human cancers. |
Immunogen: A synthetic peptide surround the 777A codon of ErbB2 protein. |
Tested Applications: ELISA, WB, IF, IHC |
Recommended Dilutions: ELISA: 1:500-1:5000 WB: 1:200-1:2000 IF: 1:100 IHC: 1:50-1:100 |
Concentration: 0.2 mg/ml |
Host Species: Mouse |
Format: Liquid |
Clonality: Monoclonal |
Isotype: IgG |
Purity: Purified from ascites |
Preservative: No |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: Recognizes V777A mutant but not wild type ErbB2 protein, of vertebrates. |
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing. |
Immunofluorescence: Immunofluorescence of cells expressing ErbB2 proteins with Anti-ErbB2(V777A) antibody. HEK293T cells were transfected with pCDNA3-GFP- ErbB2 WT plasmid (left column) or pCDNA3-GFP-ErbB2(V777A) plasmid (right column), then fixed and stained with Anti-ErbB2(V777A) monoclonal antibody (Cat. #26080). |