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ERBB2(V777A)

Anti-ErbB2(V777A) Mouse Monoclonal Antibody
5/5

$495.00

Cat.#:  26080

   Size:   100 μl

In Stock

            Product Description          
ErbB2(V777A)
Cat. #:  26080
Gene Symbol:  ERBB2/HER2/Neu
Description:   Anti-ErbB2(V777A) Mouse Monoclonal Antibody
Background:   The ErbB family is composed of four plasma membrane-bound receptor tyrosine kinases. Amplification or over-expression of the ERBB2 gene occurs in approximately 30% of breast cancers. HER2 is encoded by ERBB2, a known proto-oncogene located at the long arm of human chromosome 17(17q21-q22). HER2 proteins have been shown to form clusters in cell membranes that may play a role in tumorigenesis. Mutations of ErbB2 protein, including V777A, have been indicated in human cancers.
Immunogen:   A synthetic peptide surround the 777A codon of ErbB2 protein.
Tested Applications:   ELISA, WB, IF, IHC
Recommended Dilutions:    ELISA: 1:500-1:5000 WB: 1:200-1:2000 IF: 1:100 IHC: 1:50-1:100
Concentration:   0.2 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Preservative: No
Constituents:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes V777A mutant but not wild type ErbB2 protein, of vertebrates.
Storage Conditions:  Store at -20°C. Avoid repeated freezing and thawing.
Immunofluorescence:   Immunofluorescence of cells expressing ErbB2 proteins with Anti-ErbB2(V777A) antibody. HEK293T cells were transfected with pCDNA3-GFP- ErbB2 WT plasmid (left column) or pCDNA3-GFP-ErbB2(V777A) plasmid (right column), then fixed and stained with Anti-ErbB2(V777A) monoclonal antibody (Cat. #26080).
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