NewEast Biosciences pioneered the research and development of the antibodies for GTPases and mutated Oncogene ten years ago. GTPases involve (1) signal transduction in response to activation of cell surface receptors, including transmembrane receptors such as those mediating taste, smell and vision, (2) protein biosynthesis at the ribosome, (3) regulation of cell differentiation, proliferation, division and movement, (4) translocation of proteins through membranes, (5) transport of vesicles within the cell, and vesicle-mediated secretion and uptake, through GTPase control of vesicle coat assembly. An oncogene is a gene that has the potential to cause cancer.
We offer three unique categories of antibodies, which (1) recognize only the active configuration of GTPase (not the inactive one), (2) mutated Oncogene (not mild type) and (3) have super affinity for cAMP and cGMP (no acetylation required). We have over one thousand peer reviewed articles cited our products.
$495.00
Cat. #: 26249 |
Gene Symbol: FGFR1, BFGFR, CD331, CEK, FGFBR, FLG |
Description: Anti-FGFR1(P252R) Mouse Monoclonal Antibody |
Background: The protein FGFR1 is highly conserved between members and throughout evolution. This particular family member of FGFR1 binds both acidic and basic fibroblast growth factors and is involved in limb induction. Mutations of FGFR1 protein have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, squamous cell lung cancer and autosomal dominant Kallmann syndrome. |
Immunogen: A synthetic peptide from the internal region of FGFR1 which includes the mutation of P252R, human origin. |
Applications: ELISA, WB, IHC |
Recommended Dilutions: ELISA: 1:1000-1:2000 WB: 1:500-1:1000 IHC: 1:50-1:100 |
Concentration: 1 mg/ml |
Host Species: Mouse |
Format: Liquid |
Clonality: Monoclonal |
Isotype: IgG |
Purity: Purified from ascites |
Preservative: No |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: Recognizes P252R mutant, but not wild type FGFR1 of vertebrates. |
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot: Western blot analysis of recombinant FGFR1(P252R) and wild type proteins. Purified His-tagged FGFR1(P252R) protein (22-376aa, 45KD, lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-FGFR1(P252R) monoclonal antibody (Cat. #26249). Immunofluorescence: Immunofluorescence of cells expressing FGFR1 proteins with Anti-FGFR1(P252R) antibody. HEK293T cells were transfected with pCDNA3-GFP-FGFR1 (WT) plasmid (left column) or pCDNA3-GFP-FGFR1(P252R) plasmid (right column), then fixed and stained with Anti-FGFR1(P252R) monoclonal antibody (Cat. #26249). |