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Human FZD7 Full Length Protein

Human FZD7 Full Length Protein-Synthetic Nanodisc
5/5

$4,400.00

Cat.#:  11090

   Size:   50 μg

In Stock

          Product Description          
Cat.#:     11090
Product Name:   Human FZD7 Full Length Protein
Size :    10 µg; 50 µg and 100 µg
Synonyms:   FzE3
Target:    FZD7
UNIPROT ID:    O75084
Description:    Human FZD7 Full Length Protein-Synthetic Nanodisc
Background:     Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD7 protein contains an N-terminal signal sequence, 10 cysteine residues typical of the cysteine-rich extracellular domain of Fz family members, 7 putative transmembrane domains, and an intracellular C-terminal tail with a PDZ domain-binding motif. FZD7 gene expression may downregulate APC function and enhance beta-catenin-mediated signals in poorly differentiated human esophageal carcinomas.
Species/Host:    HEK293
Molecular Weight:    The human full length FZD7 protein has a MW of 63.6 kDa
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
Protein Families:    Druggable Genome, Transmembrane
Protein Pathways:    Basal cell carcinoma, Colorectal cancer, Melanogenesis, Pathways in cancer, Wnt signaling pathway

Figure 1. Elisa plates were pre-coated with Flag Tag FZD7-Nanodisc (0.2μg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with FZD7-Nanodisc is 1.783ng/ml.

Figure 2. Human FZD7-Nanodisc, Flag Tag on SDS-PAGE
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