NewEast Biosciences pioneered the research and development of the antibodies for GTPases and mutated Oncogene ten years ago. GTPases involve (1) signal transduction in response to activation of cell surface receptors, including transmembrane receptors such as those mediating taste, smell and vision, (2) protein biosynthesis at the ribosome, (3) regulation of cell differentiation, proliferation, division and movement, (4) translocation of proteins through membranes, (5) transport of vesicles within the cell, and vesicle-mediated secretion and uptake, through GTPase control of vesicle coat assembly. An oncogene is a gene that has the potential to cause cancer.
We offer three unique categories of antibodies, which (1) recognize only the active configuration of GTPase (not the inactive one), (2) mutated Oncogene (not mild type) and (3) have super affinity for cAMP and cGMP (no acetylation required). We have over one thousand peer reviewed articles cited our products.
$239.00
Gα13 Monoclonal Antibody
Cat. #: 26004 |
Gene Symbol: Gna13 |
Description: Anti-Gα13 Mouse Monoclonal Antibody |
Background: Heterotrimeric G proteins are essential cellular signal transducers. Gα13 is one of the G proteins that could mediate cell migration and angiogenesis. Other biochemical and physiological functions of Gα13 are being explored. |
Immunogen: Recombinant full length Gα13 protein |
Applications: ELISA, WB, IHC |
Recommended Dilutions: ELISA 1:1000-1:5000, WB 1:1000-1:2000 |
Concentration: 1 mg/ml |
Host Species: Mouse |
Format: Liquid |
Clonality: Monoclonal |
Isotype: IgG |
Purity: Purified from ascites |
Preservative: No |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: Gα13 monoclonal antibody recognizes Gα13 of vertebrates. |
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot: Gα13, Gα12 or Gαq plasmid DNAs were transiently transfected into CHO cells (endogenous Gα13 protein was down-regulated by Gα13siRNA). Whole-cell lysates were used for western blots. |
1. PLEXIN-B2 promotes the osteogenic differentiation of human bone marrow mesenchymal stem cells via activation of the RhoA signaling pathway Cell Signal. 2019 PMID: 31176746 |
2. The interaction of Ga13 with integrin ß1 mediates cell migration by dynamic regulation of RhoA Mol Biol Cell. 2015 PMID: 26310447 |
3. B-lymphocyte Specific loss of Ric-8A Results in a Gα Protein Deficit and Severe Humoral Immunodeficiency J Immunol. 2015 PMID: 26232433 |
4. Evidence of a common mechanism of disassembly of adherens junctions through Ga13 targeting of VE-cadherin J Exp Med. 2014 PMID: 24590762 |
5. A directional switch of integrin signaling and a new anti-thrombotic strategy Nature. 2013 PMID: 24162846 |
6. Suppression of Tumor Angiogenesis by Ga13 Haploinsufficiency J Biol Chem. 2009 PMID: 19654325 |