NewEast Biosciences pioneered the research and development of the antibodies for GTPases and mutated Oncogene ten years ago. GTPases involve (1) signal transduction in response to activation of cell surface receptors, including transmembrane receptors such as those mediating taste, smell and vision, (2) protein biosynthesis at the ribosome, (3) regulation of cell differentiation, proliferation, division and movement, (4) translocation of proteins through membranes, (5) transport of vesicles within the cell, and vesicle-mediated secretion and uptake, through GTPase control of vesicle coat assembly. An oncogene is a gene that has the potential to cause cancer.
We offer three unique categories of antibodies, which (1) recognize only the active configuration of GTPase (not the inactive one), (2) mutated Oncogene (not mild type) and (3) have super affinity for cAMP and cGMP (no acetylation required). We have over one thousand peer reviewed articles cited our products.
$219.00
Cat. #: 21006 |
Gene Symbol: Gα i |
Description: Anti-Gα i Rabbit Polyclonal Antibody |
Background: Heterotrimeric G proteins are critical cellular signal transducers. Gα irepresents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gα i proteins are being explored. |
Immunogen: Recombinant full length protein of Gα i1 |
Applications: ELISA, WB, IHC |
Recommended Dilutions: ELISA 1:100-1:5000, WB 1:100-1:2000 |
Concentration: 1 mg/ml |
Host Species: Rabbit |
Format: Liquid |
Clonality: Polyclonal |
Isotype: IgG |
Purity: Purified from serum |
Preservative: No |
Constituents: PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: Gα i antibody recognizes Gα i1, Gα i2, and Gα i3 of vertebrates |
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot: Western blot analysis of Gi proteins. Gα i1 plasmid DNA was transiently transfected into HEK-293 cells. Whole-cell lysate was used for western blot with the polyclonal anti Gα i antibody (cat # 21006). |