$495.00
| Anti GαoGTP Mouse Monoclonal Antibody |
| Cat. #: 26907 |
| Size: 30 µL |
| Gene Symbol: Gnao |
| Description: Anti Gαo-GTP Mouse Monoclonal Antibody |
| Background: Heterotrimeric G proteins are critical cellular signal transducers. Gαo represents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gαo proteins are being explored. |
| Immunogen: Recombinant full length protein of active Gαo |
| Applications: IP, IHC and IF (Not applicable for WB since SDS denatures Gαo GTPase) |
| Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG1 |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Anti-active Gαo antibody recognizes active Gαo of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
| Immunoprecipitation/Western blot:
Gαo Activation Assay.
Purified Gαo proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the anti-active Gαo monoclonal antibody (Cat. No. 26907). Immunoblot was with an anti-Gαopolyclonal antibody (Cat. No. 21015). |
| 1. The Inhibitory Role of Hydrogen Sulfide in UII-Induced Cardiovascular Effects and the Underlying Signaling Pathways Antioxidants (Basel). 2022 PMID: 36421438 |
| 2. Golgi-Resident Gao Promotes Protrusive Membrane Dynamics Cell. 2017 PMID: 28803726 |
| 3. The Gao Activator Mastoparan-7 Promotes Dendritic Spine Formation in Hippocampal Neurons Neural Plast. 2016 PMID: 26881110 |
| 4. Wnt-5a/Frizzled9 Receptor Signaling through the Gαo-Gβγ Complex Regulates Dendritic Spine Formation J Biol Chem. 2016 PMID: 27402827 |
| 5. Toll-like receptor 2-mediated MAPKs and NF-?B activation requires the GNAO1-dependent pathway in human mast cells Integr Biol (Camb). 2016 PMID: 27515449 |
| 6. Go is required for the release of IL-8 and TNF-a, but not degranulation in human mast cells Eur J Pharmacol. 2016 PMID: 27025291 |
| 7. AKT-independent Reelin signaling requires interactions of heterotrimeric Go and Src Biochem Biophys Res Commun. 2015 PMID: 26441085 |
