NewEast Biosciences pioneered the research and development of the antibodies for GTPases and mutated Oncogene ten years ago. GTPases involve (1) signal transduction in response to activation of cell surface receptors, including transmembrane receptors such as those mediating taste, smell and vision, (2) protein biosynthesis at the ribosome, (3) regulation of cell differentiation, proliferation, division and movement, (4) translocation of proteins through membranes, (5) transport of vesicles within the cell, and vesicle-mediated secretion and uptake, through GTPase control of vesicle coat assembly. An oncogene is a gene that has the potential to cause cancer.
We offer three unique categories of antibodies, which (1) recognize only the active configuration of GTPase (not the inactive one), (2) mutated Oncogene (not mild type) and (3) have super affinity for cAMP and cGMP (no acetylation required). We have over one thousand peer reviewed articles cited our products.
$219.00
Cat. #: 21014 |
Gene Symbol: Gnaq |
Description: Anti-Gaq Rabbit Polyclonal Antibody |
Background: Heterotrimeric G proteins are essential cellular signal transducers. Gaq is a member of the Gq subfamily of G proteins. Gaq is known to stimulate phospholipase C. Other biochemical and physiological functions of Gaq are being explored. |
Immunogen: Recombinant full length Gaq protein |
Applications: ELISA, WB, IHC |
Recommended Dilutions: ELISA 1:1000-1:5000 WB 1:100-1:2000 |
Concentration: 1 mg/ml |
Host Species: Rabbit |
Format: Liquid |
Clonality: Polyclonal |
Isotype: IgG |
Purity: Purified from serum |
Preservative: No |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: Gaq polyclonal antibody recognizes Gaq of vertebrates. |
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot: Western blot analysis of Gaq proteins. MEF whole-cell lysates were used for western blot with Anti-Gaq polyclonal antibody (cat # 21014). |
1. A G protein-coupled a7 nicotinic receptor regulates signaling and TNF-a release in microglia FEBS Open Bio. 2017 PMID: 28904864 |
2. Alpha 7 nicotinic receptor coupling to heterotrimeric G proteins modulates RhoA activation, cytoskeletal motility, and structural growth J Neurochem. 2016 PMID: 27167578 |
3. Identification and Characterization of a G Protein-binding Cluster in α7 Nicotinic Acetylcholine Receptors J Biol Chem. 2015 PMID: 26088141 |
4. Muscarinic acetylcholine receptor-mediated activation of G(q) in rat brain membranes determined by guanosine-5′-O-(3-[35S]thio)triphosphate ([35S]GTPgammaS) binding using an anti-G protein scintillation proximity assay J Neural Transm. 2012 PMID: 22127550 |