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q(Q209R)

Anti-GNAQ(Q209R) Mouse Monoclonal Antibody
5/5

$495.00

Cat.#:  26330

   Size:   100 μl

In Stock

          Product Description          

q(Q209R)

Cat. #:  26330
Gene Symbol:  GNAQ, CMC1, G-ALPHA-q, GAQ, SWS
Description:   Anti-GNAQ(Q209R) Mouse Monoclonal Antibody
Background:   GNAQ is a protein that in humans encoded by the GNAQ gene. The GNAQ protein, an alpha subunit in the Gq class, couples a seven-transmembrane domain receptor to activation of phospholipase C-beta. Mutations at this locus have been associated with problems in platelet activation and aggregation.
Immunogen:   A synthetic peptide from the internal region of GNAQ which includes the mutation of Q209R, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   ELISA: 1:1000-1:2000 WB: 1:500-1:1000 IHC: 1:50-1:100
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Preservative: No
Constituents:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes Q209R mutant, but not wild type GNAQ of vertebrates.
Storage Conditions:   Store at -20°C. Avoid repeated freezing and thawing

Western blot:  

Western blot analysis of recombinant GNAQ(Q209R) and wild type proteins.

Purified His-tagged GNAQ(Q209R) protein (lane2) and corresponding wild type protein (lane1) were blotted with Anti-GNAQ(Q209R) monoclonal antibody (Cat. #26330).

Immunofluorescence:  

Immunofluorescence of cells expressing GNAQ proteins with Anti-GNAQ(Q209R) antibody.

HEK293T cells were transfected with pCDNA3-GFP-GNAQ(Q209R) plasmid, pCDNA3-GFP-GNAQ (WT) plasmid, pCDNA3-GFP-GNAQ (Q209L) plasmid or pCDNA3-GFP-GNAQ (Q209P) plasmid, then fixed and stained with Anti-GNAQ(Q209R) monoclonal antibody (Cat. #26330).

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