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Human GPR20 Full Length Protein

Human GPR20 Full Length Protein-Synthetic Nanodisc


Cat.#:  11089

   Size:   50 μg

3-4 weeks

          Product Description          
Cat.#:     11089
Product Name:   Human GPR20 Full Length Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   ——
Target:    GPR20
UNIPROT ID:    Q99678
Description:    Human GPR20 full length protein-synthetic nanodisc
Background:     GPR20 is one of the orphan GPCRs that has been identified from human genomic DNA by PCR amplification using primers based on the sequences of the opioid/somatostatin-related receptors, GPR7 and GPR8. The expression of human GPR20 has been detected in several brain regions, including the caudate nuclei, putamen, and the thalamus. A recently disclosed patent demonstrated that GPR20-deficient mice exhibited a hyperactivity disorder characterized by an increase in total distance traveled in an open field test, implying a substantial role of GPR20 in neurophysiological function. However, the physiological mechanisms of GPR20 action, including the identification of natural ligands for GPR20, have not yet been elucidated.
Species/Host:    HEK293
Molecular Weight:    The human full length GPR20 protein has a MW of 38.7 kDa
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure1. Elisa plates were pre-coated with Flag Tag GPR20-Nanodisc (0.2 µg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with GPR20-Nanodisc is 4.663ng/ml.

Figure2. Human GPR20-Nanodisc, Flag Tag on SDS-PAGE