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Human GPR84 Full Length Protein

Human GPR84 Full Length Protein-Synthetic Nanodisc
5/5

$4,400.00

Cat.#:  11110

   Size:   50 μg

In Stock

          Product Description          
Cat.#:     11110
Product Name:   Human GPR84 Full Length Protein
Size :    10 µg; 50 µg and 100 µg
Synonyms:   EX33; GPCR4
Target:    GPR84
UNIPROT ID:    Q9NQS5
Description:    Human GPR84 Full Length Protein-Synthetic Nanodisc
Background:     Receptor for medium-chain free fatty acid (FFA) with carbon chain lengths of C9 to C14. Capric acid (C10:0), undecanoic acid (C11:0) and lauric acid (C12:0) are the most potent agonists. Not activated by short-chain and long-chain saturated and unsaturated FFAs. Activation by medium-chain free fatty acid is coupled to a pertussis toxin sensitive G(i/o) protein pathway. May have important roles in processes from fatty acid metabolism to regulation of the immune system.
Species/Host:    HEK293
Molecular Weight:    The human full length GPR84 protein has a MW of 43.7 kDa
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
Protein Families:    Druggable Genome, GPCR, Transmembrane
Protein Pathways:    N/A

Figure 1. Elisa plates were pre-coated with Flag Tag GPR84-Nanodisc (0.2μg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with GPR84-Nanodisc is 2.893ng/ml. 

Figure 2. WB analysis of Human GPR84-Nanodisc with anti-Flag monoclonal antibody at 1/5000 dilution, followed by Goat Anti-Rabbit IgG HRP at 1/5000 dilution
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