| Cat.#: 11080 |
| Product Name: Human HCRTR1 Full Length Protein |
| Size : 10 µg; 50 µg and 100 µg |
| Synonyms: ORXR1;OX1R;OXR1 |
| Target: HCRTR1 |
| UNIPROT ID: O43613 |
| Description: Human HCRTR1 Full Length Protein-Synthetic Nanodisc |
| Background: The protein is a G-protein coupled receptor involved in the regulation of feeding behavior. The encoded protein selectively binds the hypothalamic neuropeptide orexin A. A related gene (HCRTR2) encodes a G-protein coupled receptor that binds orexin A and orexin B. |
| Species/Host: HEK293 |
| Molecular Weight: The human full length HCRTR1 protein has a MW of 47.5 kDa |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Protein Families: Druggable Genome, ES Cell Differentiation/IPS, GPCR, Transmembrane |
| Protein Pathways: Neuroactive ligand-receptor interaction |
Figure 1. Elisa plates were pre-coated with Flag Tag HCRTR1-Nanodisc (0.2μg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with HCRTR1-Nanodisc is 4.139ng/ml. | 
Figure 2. WB analysis of Human HCRTR1-Nanodisc with anti-Flag monoclonal antibody at 1/5000 dilution, followed by Goat Anti-Rabbit IgG HRP at 1/5000 dilution |
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