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Human High Density Lipoprotein


Cat.#:  10460

   Size:   2.0 mg

One Week Lead Time

          Product Description          
Cat. #: 10460
Name: 2.0 mg
Purity: 98% (Co-migrates with reference on agarose gel electrophoresis)
Concentration: Minimum 2.25 mg/ml protein
Description: Human High Density Lipoprotein
Background:   LDL is a large protein (MW 3,500 kDa) with a diameter of 25.8 nm. It is composed of approximately 20-25% protein and 75-80% lipid. The lipid portion can be further described as 9% free cholesterol, 42% cholesteryl ester, 20-24% phospholipid, and 5% triglyceride.  
Source: Human plasma, which tested negative for HBsAG, HIV1/2, HIV-NAT, HCV, HCV-NAT and Syphilis. It is purified via ultracentrifugation to homogeneity (d=1.063~1.21 g/mL) determined by agarose gel electrophoresis. Each lot is analyzed on agarose gel electrophoresis for migration versus HDL.
Tested Applications: Human HDL are evaluated for receptor binding to Activity: peritoneal macrophages in conjunction with our DiI-HDL and [I-125] Ox-HDL.
Storage & Stability: Human HDL is stable for 6 weeks after receipt when handled aseptically and stored at 2-8°C (Don’t Freeze). Note: After prolonged storage, some precipitate may be observed. This is normal for the product. Spin in centrifugation at 1000×g for 3 minutes before using.
Packaging: HDL is membrane filtered (0.22 micron) and aseptically packaged under nitrogen in a solution containing phosphate-buffered saline at pH 7.4 and 0.2 mM EDTA-Na2. The product requires 1 week lead time.  Please plan your experiments in advance and use the fresh material.
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