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His Tag (DM1) Rabbit mAb

Anti-His Tag (DM1) Rabbit Monoclonal Antibody


Cat.#:  28287

   Size:   100 μg

In Stock

          Product Description          
Cat.#:   28287
Product Name:   Anti-His Tag(DM1) Rabbit Monoclonal Antibody
Synonyms:   his tag
Description:    Anti-His Tag Antibody(DM1) Rabbit Monoclonal Antibody
Background:    The rabbit immune system generates antibody diversity and optimizes affinity. Anti-His Tag Antibody(DM1); Rabbit mAb is specific to six histidine tags placed at C-terminal and internal regions of fusion proteins. This antibody can greatly improve the effectiveness of several different kinds ofimmunoassays; helping researchers identify; detect; and purify polyhistidine fusion proteinsin bacteria; insect cells; and mammalian cells.
Applications:    ELISA; WB
Recommended Dilutions:    WB 1:1000
Host Species:    Rabbit
Isotype:    Rabbit IgG
Purification:    Purified from cell culture supernatant by affinity chromatography
Species Reactivity:    Detects proteins containing accessible consecutive histidine regions. The antibody detects His tags localized at the amino- or carboxyl- terminus. His Tag
Constituents:    Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).

Figure 1. Western blot analysis of his-tag fusion protein (70kDa, 20ng) using His-Tag antibody (28287) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:5000 dilution.