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Human ADGRE2 Protein, His Tag

Recombinant human ADGRE2 protein with C-terminal 7?His tag


Cat.#:  11530

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     11530
Product Name:   Human ADGRE2 Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   Adhesion G protein-coupled receptor E2;GF-like module receptor 2;GF-like module-containing mucin-like hormone receptor-like 2;D312
Target:    ADGRE2
Description:    Recombinant human ADGRE2 protein with C-terminal 7xHis tag
Background:     This gene encodes a member of the class B seven-span transmembrane (TM7) subfamily of G-protein coupled receptors. These proteins are characterized by an extended extracellular region with a variable number of N-terminal epidermal growth factor-like domains coupled to a TM7 domain via a mucin-like spacer domain. The encoded protein is expressed mainly in myeloid cells where it promotes cell-cell adhesion through interaction with chondroitin sulfate chains. This gene is situated in a cluster of related genes on chromosome 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 57.2 kDa after removal of the signal peptide. The apparent molecular mass of ADGRE2-His is approximately 70-100 kDa due to glycosylation.
Molecular Characterization:    ADGRE2(Gln24-Thr537) 7×His tag
Purity:    The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human ADGRE2 Protein, His Tag on SDS-PAGE under reducing condition.