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Human AXL Protein, His Tag

Recombinant human AXL protein with C-terminal 6XHis tag
5/5

$479.00

Cat.#:  11193

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     11193
Product Name:   Human AXL Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   AXL;FO
Target:    AXL
UNIPROT ID:    P30530
Description:    Recombinant human AXL protein with C-terminal 6xHis tag
Background:     The protein encoded by this gene is a member of the Tyro3-Axl-Mer (TAM) receptor tyrosine kinase subfamily. The encoded protein possesses an extracellular domain which is composed of two immunoglobulin-like motifs at the N-terminal, followed by two fibronectin type-III motifs. It transduces signals from the extracellular matrix into the cytoplasm by binding to the vitamin K-dependent protein growth arrest-specific 6 (Gas6). This gene may be involved in several cellular functions including growth, migration, aggregation and anti-inflammation in multiple cell types. Alternative splicing results in multiple transcript variants of this gene.
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 46.6 kDa after removal of the signal peptide.
Molecular Characterization:    AXL(Ala26-Pro449) 6×His tag
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human AXL Protein, His Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human AXL, His tagged protein (11193) can bind Anti-AXL Antibody 28042 in a linear range of 0.64-80.0 ng/ml.

Figure 3. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human AXL, His tagged protein (11193) can bind Human NTD, mFc Tagged protein 11298 in a linear range of 0.006-0.16 µg/ml.

Figure 4. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human AXL, His tagged protein (11193) can bind Human NTD, hFc Tagged protein 11299 in a linear range of 0.0012-0.16 µg/ml.
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