Email:   |  Call: 6109452007

Human B7-H5 Protein, hFc Tag

Recombinant Human B7-H5 with C-terminal human Fc tag


Cat.#:  11729

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     11729
Product Name:   Human B7-H5 Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   B7-H5;B7H5;C10orf54;DD1alpha;Dies1;GI24;PD-1H;PP2135;SISP1;VISTA
Target:    B7-H5
Description:    Recombinant Human B7-H5 with C-terminal human Fc tag
Background:     Immunoregulatory receptor which inhibits the T-cell response (PubMed:24691993). May promote differentiation of embryonic stem cells, by inhibiting BMP4 signaling (By similarity). May stimulate MMP14-mediated MMP2 activation (PubMed:20666777).[UniProtKB/Swiss-Prot Function]
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 44.3 kDa after removal of the signal peptide.The apparent molecular mass of B7-H5-hFc is approximately 55-70 kDa due to glycosylation.
Molecular Characterization:    B7-H5(Phe33-Ala194) hFc(Glu99-Ala330)
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human B7-H5 Protein, hFc Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human B7-H5 Protein, hFc Tag (11729) can bind Anti-B7-H5 Neutralizing antibody 28108 in a linear range of 2.44-625.00 ng/mL.