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Human BTN3A1 Protein, mFc-His Tag

Recombinant Human BTN3A1 protein with C-terminal mouse Fc and 6XHis tag


Cat.#:  11180

   Size:   100 μg

In Stock

            Product Description          
Cat.#:     11180
Product Name:   Human BTN3A1 Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   BTN3A1;BTF5;CD277;BTN3.1;BT3.1
Target:    BTN3A1
UNIPROT ID:    O00481
Description:    Recombinant Human BTN3A1 protein with C-terminal mouse Fc and 6xHis tag
Background:     The butyrophilin (BTN) genes are a group of major histocompatibility complex (MHC)-associated genes that encode type I membrane proteins with 2 extracellular immunoglobulin (Ig) domains and an intracellular B30.2 (PRYSPRY) domain. Three subfamilies of human BTN genes are located in the MHC class I region: the single-copy BTN1A1 gene (MIM 601610) and the BTN2 (e.g., BTN2A1, MIM 613590) and BTN3 (e.g., BNT3A1) genes, which have undergone tandem duplication, resulting in 3 copies of each.
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 51.36 kDa after removal of the signal peptide.
Molecular Characterization:    BTN3A1(Glu30-Gly254) mFc(Pro99-Lys330) 6×His tag
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human BTN3A1 Protein, mFc-His Tag on SDS-PAGE under non-reducing (NR) and reducing (R) conditions.

Figure 2. ELISA plate pre-coated by 1 µg/ml (100 µl/well) Human BTN3A1 protein, mFc-His Tag (11180) can bind Anti-BTN3A1 Rabbit mAb in a linear range of 3.2-16 ng/mL.