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Human CD123 Protein, hFc-His Tag

Recombinant human CD123 protein with C-terminal human Fc and 6XHis tag


Cat.#:  11131

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     11131
Product Name:   Human CD123 Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   IL3R;IL3RA;IL-3Ra;IL-3R-alpha;IL3RAY;IL3RX;IL3RY;CD123 antigen;CD123;hIL3Ra;hIL-3Ra;MGC34174;IL-3 R alpha
Target:    CD123
UNIPROT ID:    P26951
Description:    Recombinant human CD123 protein with C-terminal human Fc and 6xHis tag
Background:     Interleukin 3 receptor alpha (low affinity) (IL3RA), also known as CD123 (Cluster of Differentiation 123) is a 70-kD glycoprotein member of the hematopoietin receptor superfamily. This protein associates with a beta subunit common to the receptors for IL-5 and granulocyte-macrophage colony-stimulating factor (GM-CSF) to form a high-affinity receptor for IL-3. The interleukin-3 receptor a chain (CD123) has been identified as a potential immunotherapeutic target because it is overexpressed in AML compared with normal hematopoietic stem cells
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 80-90 kDa after removal of the signal peptide.
Molecular Characterization:    CD123(Thr19-Arg305) hFc(Glu99-Ala330) 6×His tag
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human CD123, hFc-His Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CD123, hFc-His tagged protein (11131) can bind Anti-CD123 Neutralizing antibody 28012 in a linear range of 0.98-26.70 ng/ml.