| Cat.#: 11155 |
| Product Name: Human CD155 Protein |
| Size : 10 µg, 50 µg and 100 µg |
| Synonyms: PVR;FLJ25946;PVS;CD155;TAGE4;HVED;NECL5 |
| Target: CD155 |
| UNIPROT ID: P15151 |
| Description: Recombinant human CD155 protein with C-terminal mouse Fc and 6xHis tag |
| Background: The protein encoded by this gene is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. The external domain mediates cell attachment to the extracellular matrix molecule vitronectin, while its intracellular domain interacts with the dynein light chain Tctex-1/DYNLT1. The gene is specific to the primate lineage, and serves as a cellular receptor for poliovirus in the first step of poliovirus replication. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Species/Host: HEK293 |
| Molecular Weight: The protein has a predicted molecular mass of 62.2 kDa after removal of the signal peptide. The apparent molecular mass of CD155-mFc-His is approximately 95-130 kDa due to glycosylation. |
| Molecular Characterization: CD155(Trp21-Asn343) mFc(Pro99-Lys330) 6×His tag |
| Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Figure 1. Human CD155 Protein, mFc-His Tag on SDS-PAGE under reducing condition. | 
Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CD155, mFc-His tagged protein (11155) can bind Human TIGIT, hFc tagged protein 11225 in a linear range of 1.95-125 ng/ml. | 
Figure 3. ELISA plate pre-coated by 1 µg/ml (100 µl/well) Human CD155 Protein, mFc-His Tag(11155) can bind Anti-CD155 Rabbit mAb in a linear range of 3.2-80 ng/mL. | |
|
