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Human CD200 Protein, His Tag

Recombinant human CD200 protein with C-terminal 6XHis tag


Cat.#:  11278

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     11278
Product Name:   Human CD200 Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   CD200;OX1;OX2;RC;X-2;y033
Target:    CD200
UNIPROT ID:    P41217
Description:    Recombinant human CD200 protein with C-terminal 6xHis tag
Background:     This gene encodes a type I membrane glycoprotein containing two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. This gene is expressed by various cell types, including B cells, a subset of T cells, thymocytes, endothelial cells, and neurons. The encoded protein plays an important role in immunosuppression and regulation of anti-tumor activity. Alternative splicing results in multiple transcript variants encoding different isoforms.
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 23.3 kDa after removal of the signal peptide.
Molecular Characterization:    CD200(Gln31-Gly232) 6×His tag
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human CD200 Protein, His Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CD200 Protein, His Tag (11278) can bind Anti-CD200 antibody(DM156), Rabbit mAb in a linear range of 0.64-3.20 ng/mL.