Cat.#: 11142 |
Product Name: Human CD28 Protein |
Size : 10 µg, 50 µg and 100 µg |
Synonyms: CD28;Tp44 |
Target: CD28 |
UNIPROT ID: P10747 |
Description: Recombinant human CD28 protein with C-terminal mouse Fc and 6xHis tag |
Background: The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
Species/Host: HEK293 |
Molecular Weight: The protein has a predicted molecular mass of 68-72 kDa after removal of the signal peptide. |
Molecular Characterization: CD28(Asn19-Pro152) mFc(Pro99-Lys330) 6×His |
Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
 Figure 1. Human CD28 Protein, mFc-His Tag on SDS-PAGE under reducing condition. |  Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human B7-1, hFc tagged protein 11283 can bind Human CD28, mFc-His tagged protein (11142) in a linear range of 125-4000 ng/ml. |  Figure 3. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human B7-2, hFc tagged protein 11284 can bind Human CD28, mFc-His tagged protein (11142) in a linear range of 62.5-4000 ng/ml. |  Figure 4. HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28, mFc-His tagged protein (11142) 1µg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody. |
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