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Human CD30 Protein, His tag

Recombinant human CD30 protein with C-terminal 6XHis tag
5/5

$555.00

Cat.#:  11291

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     11291
Product Name:   Human CD30 Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   TNFRSF8;D30;1S166E;i-1
Target:    CD30
UNIPROT ID:    P28908
Description:    Recombinant human CD30 protein with C-terminal 6xHis tag
Background:     The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 39.3 kDa after removal of the signal peptide. The apparent molecular mass of CD30-His is approximately 55-70 kDa due to glycosylation.
Molecular Characterization:    CD30(Phe19-Lys379) 6×His tag
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human CD30 Protein, His Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CD30, His tagged protein (11291) can bind Anti-CD30 Neutralizing antibody 28026 in a linear range of 0.13-80.0 ng/ml.

Figure 3. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CD30, His tagged protein (11291) can bind Human CD30 Ligand,mFc-His tagged protein 11167 in a linear range of 2.56-320 ng/ml.

Figure 4. Flow cytometry analysis with 1 µg/ml Human CD30 Protein, His tag (11291) on Expi293 cells transfected with human CD30L (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
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