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Human CD33(140-259) Protein, hFc Tag

Recombinant human CD33 protein with C-terminal human Fc tag
5/5

$389.00

Cat.#:  11489

   Size:   100 μg

In Stock

            Product Description          
Cat.#:     11489
Product Name:   Human CD33(140-259) Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   CD33;IGLEC3;p67
Target:    CD33
UNIPROT ID:    P20138
Description:    Recombinant human CD33 protein with C-terminal human Fc tag
Background:     Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state. Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans. Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK. These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2. In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules. One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K.
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 38.9 kDa after removal of the signal peptide.
Molecular Characterization:    CD33(Asp140-His259) hFc(Glu99-Ala330)
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human CD33, hFc Tag on SDS-PAGE under reducing condition.
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