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Human CD70 Protein, mFc-His Tag

Recombinant human CD70 protein with N-terminal mouse Fc and 6XHis tag


Cat.#:  11168

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     11168
Product Name:   Human CD70 Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   CD70;D27LG;NFSF7;NFSF7G;D27L
Target:    CD70
UNIPROT ID:    P32970
Description:    Recombinant human CD70 protein with N-terminal mouse Fc and 6xHis tag
Background:     The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T and B lymphocytes. It induces proliferation of costimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin sythesis.
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 45.6 kDa after removal of the signal peptide.
Molecular Characterization:    mFc(Pro99-Lys330) CD70(Gln39-Pro193) 6×His
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human CD70 Protein, mFc-His Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CD27, hFc tagged protein 11285 can bind Human CD70,mFc-His tagged protein (11168) in a linear range of 3.2-400 ng/ml.

Figure 3. ELISA plate pre-coated by 2 µg/ml (100 µl/well) CD70,mFc-His tagged protein (11168) can bind Anti-CD70 Neutralizing antibody 28014 in a linear range of 3.91-139.9 ng/ml.

Figure 4. Flow cytometry analysis with 15 µg/ml Human CD70 Protein, mFc-His tag (11168) on Expi293 cells transfected with human CD27 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).