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Human CEACAM5 Protein, His Tag

Recombinant human CEACAM5 protein with C-terminal 6XHis tag


Cat.#:  11194

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     11194
Product Name:   Human CEACAM5 Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   CEACAM-5;D66e;EA;econium antigen 100
Target:    CEACAM5
UNIPROT ID:    P06731
Description:    Recombinant human CEACAM5 protein with C-terminal 6xHis tag
Background:     This gene encodes a cell surface glycoprotein that represents the founding member of the carcinoembryonic antigen (CEA) family of proteins. The encoded protein is used as a clinical biomarker for gastrointestinal cancers and may promote tumor development through its role as a cell adhesion molecule. Additionally, the encoded protein may regulate differentiation, apoptosis, and cell polarity. This gene is present in a CEA family gene cluster on chromosome 19. Alternative splicing results in multiple transcript variants.
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 72.2 kDa after removal of the signal peptide. The apparent molecular mass of CEACAM5-His is approximately 100-130 kDa due to glycosylation.
Molecular Characterization:    CEACAM5(Lys35-Ala685) 6×His tag
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human CEACAM5 Protein, His Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CEACAM5, His tagged protein (11194) can bind Anti-CEACAM5 Antibody 28044 in a linear range of 2.56-320 ng/ml.