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Human CTLA-4 Protein, mFc-His Tag

Recombinant human CTLA-4 protein with C-terminal mouse Fc and 6XHis tag
5/5

$555.00

Cat.#:  11145

   Size:   100 μg

In Stock

            Product Description          
Cat.#:     11145
Product Name:   Human CTLA-4 Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   CTLA4;CD152
Target:    CTLA-4
UNIPROT ID:    P16410
Description:    Recombinant human CTLA-4 protein with C-terminal mouse Fc and 6xHis tag
Background:     This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases.
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 54 kDa after removal of the signal peptide.
Molecular Characterization:    CTLA-4(Gln36-Ser161) mFc(Pro99-Lys330) 6×His
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human CTLA-4 Protein, mFc-His Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human B7-1, hFc tagged protein 11283 can bind Human CTLA4, mFc-His tagged protein (11145) in a linear range of 0.048-2.094 ng/ml.

Figure 3. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human B7-2, hFc tagged protein 11284 can bind Human CTLA4, mFc-His tagged protein (11145) in a linear range of 0.048-2.694 ng/ml.

Figure 4. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CTLA-4, mFc-His tagged protein (11145) can bind Anti-CTLA-4 Neutralizing antibody 28031 in a linear range of 0.13-16.0 ng/ml.

Figure 6.  Flow cytometry analysis with 1ug/ml Human CTLA4 Protein, mFc-His tag (11145) on Expi293 cells transfected with human B7-1 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

Figure 6.  Flow cytometry analysis with 1ug/ml Human CTLA4 Protein, mFc-His tag (11145) on Expi293 cells transfected with human B7-1 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
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