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Human DNAM-1 Protein, mFc-His Tag

Recombinant human DNAM-1 protein with C-terminal mouse Fc and 6XHis tag


Cat.#:  11176

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     11176
Product Name:   Human DNAM-1 Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   DNAM1;CD226;PTA1
Target:    DNAM-1
UNIPROT ID:    Q15762
Description:    Recombinant human DNAM-1 protein with C-terminal mouse Fc and 6xHis tag
Background:     This gene encodes a glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. The protein mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. Alternative splicing results in multiple transcript variants.
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 53.5 kDa after removal of the signal peptide.
Molecular Characterization:    DNAM-1(Glu19-Asn247) mFc(Pro99-Lys330) 6×His tag
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human DNAM-1 Protein, mFc-His Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human DNAM-1, mFc-His tagged protein (11176) can bind Human CD155, hFc Tagged protein 11293 in a linear range of 0.128-32.88 ng/ml.