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Human EGFR Protein, His Tag

Recombinant human EGFR Protein with C-terminal 6XHis tag


Cat.#:  11218

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     11218
Product Name:   Human EGFR Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   EGFR;RBB;RBB1;ER1;IG61;ENA
Target:    EGFR
UNIPROT ID:    P00533
Description:    Recombinant human EGFR Protein with C-terminal 6xHis tag
Background:     The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 69.4 kDa after removal of the signal peptide.The apparent molecular mass of EGFR-His is approximately 100-130 kDa due to glycosylation.
Molecular Characterization:    EGFR(Leu25-Ser645) 6×His tag
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human EGFR Protein, His Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human EGFR, His tagged protein (11218) can bind Anti-EGFR Antibody 28043 in a linear range of 0.64-80.0 ng/ml.