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Human GFRA3 Protein, His Tag

Recombinant human GFRA3 protein with C-terminal 6XHis tag


Cat.#:  11416

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     11416
Product Name:   Human GFRA3 Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   GDNFR3
Target:    GFRA3
UNIPROT ID:    O60609
Description:    Recombinant human GFRA3 protein with C-terminal 6xHis tag
Background:     The protein encoded by this gene is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor and a member of the GDNF receptor family. It forms a signaling receptor complex with RET tyrosine kinase receptor and binds the ligand, artemin. [provided by RefSeq, Jul 2008]
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 39.1 kDa after removal of the signal peptide. The apparent molecular mass of GFRA3-His is approximately 55-70 kDa due to glycosylation.
Molecular Characterization:    GFRA3(Asp32-Asn374) 6×His tag
Purity:    The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human GFRA3 Protein, His Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human GFRA3 Protein, His Tag (11416) can bind Anti-GFRA3 Neutralizing antibody 28094 in a linear range of 2.44-156.25 ng/mL.