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Human HER3 Protein, His Tag

Recombinant Human HER3 protein with C-terminal 6XHis tag


Cat.#:  11208

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     11208
Product Name:   Human HER3 Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   HER3;ERBB3
Target:    HER3
UNIPROT ID:    P21860
Description:    Recombinant Human HER3 protein with C-terminal 6xHis tag
Background:     This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized.
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 69.39 kDa after removal of the signal peptide.The apparent molecular mass of HER3-His is approximately 100-130 kDa due to glycosylation.
Molecular Characterization:    HER3(Ser20-Thr643) 6×His tag
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human HER3 Protein, His Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human HER3, His tagged protein (11208) can bind Human NRG1, hFc tagged protein 11763 in a linear range of 3.2-400 ng/ml.

Figure 3. ELISA plate pre-coated by 1 µg/ml (100 µl/well) Human Her3, His tagged protein (11208) can bind Anti-ErBb3 Neutralizing antibody 28065 in a linear range of 3.2-16 ng/ml.