| Cat.#: 11154 |
| Product Name: Human ICOS Protein |
| Size : 10 µg, 50 µg and 100 µg |
| Synonyms: ICOS;CD278;AILIM;Inducible T-cell costimulator |
| Target: ICOS |
| UNIPROT ID: Q9Y6W8 |
| Description: Recombinant human ICOS protein with C-terminal mouse Fc and 6xHis tag |
| Background: The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation. |
| Species/Host: HEK293 |
| Molecular Weight: The protein has a predicted molecular mass of 41.1 kDa after removal of the signal peptide. |
| Molecular Characterization: ICOS(Glu21-Lys140) mFc(Pro99-Lys330) 6×His tag |
| Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Figure 1. Human ICOS Protein, mFc-His Tag on SDS-PAGE under reducing condition. | 
Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human ICOS, mFc-His tagged protein (11154) can bind Anti-ICOS Neutralizing antibody )]in a linear range of 0.24-31.25ng/ml. |
|
