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Human IL-7 Protein

Recombinant Human Interleukin-7 is produced by our E.coli expression system and the target gene encoding Asp26XHis177 is expressed.
5/5

$525.00

Cat.#:  12031

   Size:   50 μg

In Stock

            Product Description          
Cat.#:     12031
Product Name:   Human IL-7 Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   Interleukin-7;IL-7;IL7
Target:    IL-7
UNIPROT ID:    P13232
Description:    Recombinant Human Interleukin-7 is produced by our E.coli expression system and the target gene encoding Asp26-His177 is expressed.
Background:     Human Interleukin 7 (IL-7) is a potent lymphoid cell growth factor stimulating the proliferation of lymphoid progenitors. IL7 can associate with the hepatocyte growth factor (HGF) to form a hybrid cytokine that functions as a pre-pro-B cell growth-stimulating factor. Human IL7 cDNA encodes a 177 amino acid precursor protein containing a 25 amino acid signal peptide and a 152 amino acid mature protein. Human and mouse IL7 share 65% sequence identity in the mature region and both exhibit cross-species activity. IL-7 signals via IL-7 receptor (IL7R) activating multiple pathways including JaK/STAT and PI3K/AKT, which regulate lymphocyte survival, glucose uptake, proliferation, and differentiation. IL-7 is also associated with cytoplasmic IL2-R gamma for signal transduction.
Species/Host:    E.coli
Molecular Weight:    17.5 KDa
Molecular Characterization:    Not available
Purity:    Greater than 95% as determined by reducing SDS-PAGE.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Greater than 95% as determined by reducing SDS-PAGE.

Figure 2. Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL). The ED50 for this effect is 0.02-0.08 ng/ml.

Figure 3. Loaded Human IL-7RA-His on HIS1K Biosensor, can bind Human IL-7 with an affinity constant of 15.04 nM as determined in BLI assay.

Figure 4. Loaded Human IL-7RA-Fc-His on HIS1K Biosensor, can bind Human IL-7 with an affinity constant of 18.59 nM as determined in BLI assay.

Figure 5. Loaded Mouse IL-7RA-Fc on Protein A Biosensor, can bind Human IL-7 with an affinity constant of 29.2 nM as determined in BLI assay.
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