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Human IL5 Protein, His Tag

Recombinant Human IL5 protein with C-terminal 6XHis tag
5/5

$525.00

Cat.#:  11205

   Size:   100 μg

In Stock

            Product Description          
Cat.#:     11205
Product Name:   Human IL5 Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   IL-5;TRF;Interleukin-5;EDF
Target:    IL5
UNIPROT ID:    P05113
Description:    Recombinant Human IL5 protein with C-terminal 6xHis tag
Background:     This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 13.94 kDa after removal of the signal peptide.The apparent molecular mass of IL5-His is approximately 15-25 kDa due to glycosylation.
Molecular Characterization:    IL5(Ile20-Ser134) 6×His tag
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human IL5 Protein, His Tag on SDS-PAGE under reducing condition.

Figure 2. Flow cytometry analysis with 1 µg/ml Human IL5 Protein, His Tag (11205) on Expi293 cells transfected with Human IL5RA protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

Figure 3. ELISA plate pre-coated by 5 µg/ml (100 µl/well) Human IL5 protein, His Tag (11205) can bind Human IL5RA Protein, hFc Tag 11336 in a linear range of 6.4-4000 ng/mL.
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