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Human IL5RA Protein, hFc Tag

Recombinant human L5RA protein with C-terminal human Fc tag


Cat.#:  11336

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     11336
Product Name:   Human IL5RA Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   IL5Ra;D125;L-5 R alpha
Target:    IL5RA
UNIPROT ID:    Q01344
Description:    Recombinant human L5RA protein with C-terminal human Fc tag
Background:     The protein encoded by this gene is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4. Several alternatively spliced transcript variants encoding four distinct isoforms have been reported.
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 62.3 kDa after removal of the signal peptide.The apparent molecular mass of IL5RA-hFc is approximately 70-100 kDa due to glycosylation.
Molecular Characterization:    IL5RA(Asp21-Pro338) hFc(Glu99-Ala330)
Purity:    The purity of the protein is greater than 90% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human IL5RA Protein, hFc Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 5 µg/ml (100 µl/well) Human IL5 protein, His Tag 11205 can bind Human IL5RA Protein, hFc Tag (11336) in a linear range of 6.4-4000 ng/mL.