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Human M-CSF Protein, hFc Tag

Recombinant Human M-CSF Protein with C-terminal human Fc tag


Cat.#:  11350

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     11350
Product Name:   Human M-CSF Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   CSF-1;MCSF
Target:    M-CSF
UNIPROT ID:    P09603
Description:    Recombinant Human M-CSF Protein with C-terminal human Fc tag
Background:     The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 51.3 kDa after removal of the signal peptide.The apparent molecular mass of M-CSF-hFc is approximately 55-70 kDa due to glycosylation.
Molecular Characterization:    M-CSF(Glu33-Arg255) hFc(Glu99-Ala330)
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human M-CSF Protein, hFc Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CSF1R, His tagged protein 11190 can bind Human M-CSF, hFc Tagged protein(11350) in a linear range of 0.32-200 ng/ml.

Figure 3. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human M-CSF Protein, hFc Tag ( 11350) can bind Anti-M-CSF Neutralizing antibody 28104 in a linear range of 9.77-156.25 ng/mL.

Figure 4. Flow cytometry analysis with 1 µg/ml Human M-CSF Protein, hFc tag (11350) on Expi293 cells transfected with human CSF1R (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).