Email:   |  Call: 6109452007

Human OX40 Protein, hFc-His tag

Recombinant human OX40 protein with C-terminal human Fc and 6XHis tag


Cat.#:  11150

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     11150
Product Name:   Human OX40 Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   TNFRSF4;OX40;CD134;OX40L receptor;ACT35;TXGP1L
Target:    OX40
UNIPROT ID:    P43489
Description:    Recombinant human OX40 protein with C-terminal human Fc and 6xHis tag
Background:     The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been shown to activate NF-kappaB through its interaction with adaptor proteins TRAF2 and TRAF5. Knockout studies in mice suggested that this receptor promotes the expression of apoptosis inhibitors BCL2 and BCL2lL1/BCL2-XL, and thus suppresses apoptosis. The knockout studies also suggested the roles of this receptor in CD4 T cell response, as well as in T cell-dependent B cell proliferation and differentiation.
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 47.2 kDa after removal of the signal peptide.
Molecular Characterization:    OX40(Leu29-Ala216) hFc(Glu99-Ala330) 6×His tag
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human OX40 Protein, hFc-His Tag on SDS-PAGE under reducing condition.

Figure 2. HEK293 cell line transfected with irrelevant protein  (B) and human OX40 Ligand  (A) were surface stained with Human OX40, hFc-His tagged protein (11150) 1 µg/ml followed by Alexa 488-conjugated anti-human IgG secondary antibody.

Figure 3. ELISA plate pre-coated by 1 µg/ml (100 µl/well) Human OX40 Protein, hFc-His Tag (11150) can bind Anti-OX40 Rabbit mAb in a linear range of 3.2-80 ng/mL.