| Cat.#: 11378 |
| Product Name: Human PGF Protein |
| Size : 10 µg, 50 µg and 100 µg |
| Synonyms: D12S1900;PGFL;PIGF;PLGF;PlGF-2;SHGC-10760 |
| Target: PGF |
| UNIPROT ID: P49763 |
| Description: Recombinant Human PGF with C-terminal human Fc tag |
| Background: This gene encodes a growth factor found in placenta which is homologous to vascular endothelial growth factor. Alternatively spliced transcripts encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2011] |
| Species/Host: HEK293 |
| Molecular Weight: The protein has a predicted molecular mass of 43.5 kDa after removal of the signal peptide.The apparent molecular mass of PGF-hFc is approximately 35-55 kDa due to glycosylation. |
| Molecular Characterization: PGF(Leu19-Arg170) hFc(Glu99-Ala330) |
| Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Figure 1. Human PGF Protein, hFc Tag on SDS-PAGE under reducing condition. | 
Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human PGF Protein, hFc Tag(11378) can bind Anti-PGF antibody(DM190), Rabbit mAb in a linear range of 0.80-4.00 ng/mL. |
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