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Human PPT1 Protein, His Tag

Recombinant human PPT1 protein with C-terminal 6XHis tag


Cat.#:  11792

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     11792
Product Name:   Human PPT1 Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   CLN1;PPT
Target:    PPT1
UNIPROT ID:    P50897
Description:    Recombinant human PPT1 protein with C-terminal 6xHis tag
Background:     The protein encoded by this gene is a small glycoprotein involved in the catabolism of lipid-modified proteins during lysosomal degradation. The encoded enzyme removes thioester-linked fatty acyl groups such as palmitate from cysteine residues. Defects in this gene are a cause of infantile neuronal ceroid lipofuscinosis 1 (CLN1, or INCL) and neuronal ceroid lipofuscinosis 4 (CLN4). Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Dec 2008]
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass 32.1 of kDa after removal of the signal peptide. The apparent molecular mass of PPT1-His is approximately 33-40 kDa due to glycosylation.
Molecular Characterization:    PPT1(Asp28-Gly306) 6×His tag
Purity:    The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human PPT1 Protein, His Tag on SDS-PAGE under reducing condition.