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Human PRNP Protein, hFc Tag

Recombinant human PRNP protein with C-terminal human Fc tag
5/5

$445.00

Cat.#:  11517

   Size:   100 μg

In Stock

            Product Description          
Cat.#:     11517
Product Name:   Human PRNP Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   PrP;ASCR;PrP27-30;PrP33-35C;CD230
Target:    PRNP
UNIPROT ID:    P04156
Description:    Recombinant human PRNP protein with C-terminal human Fc tag
Background:     The protein encoded by this gene is a membrane glycosylphosphatidylinositol-anchored glycoprotein that tends to aggregate into rod-like structures. The encoded protein contains a highly unstable region of five tandem octapeptide repeats. This gene is found on chromosome 20, approximately 20 kbp upstream of a gene which encodes a biochemically and structurally similar protein to the one encoded by this gene. Mutations in the repeat region as well as elsewhere in this gene have been associated with Creutzfeldt-Jakob disease, fatal familial insomnia, Gerstmann-Straussler disease, Huntington disease-like 1, and kuru. An overlapping open reading frame has been found for this gene that encodes a smaller, structurally unrelated protein, AltPrp. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 48.8 kDa after removal of the signal peptide. The apparent molecular mass of PRNP-hFc is approximately 55-70 kDa due to glycosylation.
Molecular Characterization:    PRNP(Lys23-Gly229) hFc(Glu99-Ala330)
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human PRNP Protein, hFc Tag on SDS-PAGE under reducing condition.
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