| Cat.#: 11364 |
| Product Name: Human SCF Protein |
| Size : 10 µg, 50 µg and 100 µg |
| Synonyms: DCUA;DFNA69;FPH2;FPHH;Kitl;KL-1;MGF;SCF;SF;SHEP7;SLF |
| Target: SCF |
| UNIPROT ID: P21583 |
| Description: Recombinant human SCF protein with C-terminal human Fc tag |
| Background: This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Species/Host: HEK293 |
| Molecular Weight: The protein has a predicted molecular mass of 47.2 kDa after removal of the signal peptide.The apparent molecular mass of SCF-hFc is approximately 55-75 kDa due to glycosylation. |
| Molecular Characterization: SCF(Glu26-His214) hFc(Glu99-Ala330) |
| Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Figure 1. Human SCF Protein, hFc Tag on SDS-PAGE under reducing condition. | 
Figure 2. ELISA plate pre-coated by 1 µg/ml (100 µl/well) Human CD117, His tagged protein 11321 can bind Human SCF, hFc tagged protein (11364) in a linear range of 3.2-400 ng/ml. |
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