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Human VEGFR2 Protein, His Tag

Recombinant human VEGFR2 Protein with C-terminal 6XHis tag


Cat.#:  11222

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     11222
Product Name:   Human VEGFR2 Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   CD309;FLK1;VEGFR;VEGFR2
Target:    VEGFR2
UNIPROT ID:    P35968
Description:    Recombinant human VEGFR2 Protein with C-terminal 6xHis tag
Background:     Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq, May 2009]
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 84.1kDa after removal of the signal peptide.The apparent molecular mass of VEGFR2-His is approximately 130-250 kDa due to glycosylation.
Molecular Characterization:    VEGFR2(Ala20-Glu764) 6×His tag
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human VEGFR2 Protein, His Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 5 µg/ml (100 µl/well) Human VEGFR2 protein, His Tag (11222) can bind Anti-VEGFR2 Neutralizing antibody 28067 in a linear range of 6.4-800 ng/mL.