$239.00
| Cat. #: 26136 |
| Gene Symbol: IDH1 |
| Description: Anti-IDH1 Mouse Monoclonal Antibody |
| Background: Isocitrate dehydrogenase (IDH) catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations of Arg-132 in IDH to Cys, His, Leu or Ser abolish magnesium binding and the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors. |
| Immunogen: Recombinant proteins of human IDH1 (amino acids 1-240). |
| Applications: ELISA, WB, IHC, IP |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:100-1:1000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Anti-IDH1 antibody recognizes IDH1 of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
| Western blot:
Western blot analysis of recombinant IDH1 protein. Purified His-tagged IDH1 protein (amino acids 1-240) (Cat.#10198) was blotted with anti-IDH1 mouse antibody (Cat.# 26136). |
| 1. PRL-3 is a potential glioblastoma prognostic marker and promotes glioblastoma progression by enhancing MMP7 through the ERK and JNK pathways Theranostics. 2018 PMID: 29556339 |
